Detection of Rare Mutations in EGFR-ARMS-PCR-Negative Lung Adenocarcinoma by Sanger Sequencing
نویسندگان
چکیده
منابع مشابه
Detection of Rare Mutations in EGFR-ARMS-PCR-Negative Lung Adenocarcinoma by Sanger Sequencing
PURPOSE This study aimed to identify potential epidermal growth factor receptor (EGFR) gene mutations in non-small cell lung cancer that went undetected by amplification refractory mutation system-Scorpion real-time PCR (ARMS-PCR). MATERIALS AND METHODS A total of 200 specimens were obtained from the First Affiliated Hospital of Guangzhou Medical University from August 2014 to August 2015. In...
متن کاملA novel ARMS-based assay for the quantification of EGFR mutations in patients with lung adenocarcinoma
Quantification of epidermal growth factor receptor (EGFR) mutations is important for the prediction of tyrosine kinase inhibitor (TKI) efficacy in patients with non-small cell lung cancer (NSCLC). However, clinicians lack a sensitive and convenient method to quantify EGFR mutant abundance. The present study introduces a novel method, namely amplification refractory mutation system (ARMS)-Plus, ...
متن کاملScreening PCR Versus Sanger Sequencing: Detection of CALR Mutations in Patients With Thrombocytosis
BACKGROUND Mutations in calreticulin (CALR) have been reported to be key markers in the molecular diagnosis of myeloid proliferative neoplasms. In most previous reports, CALR mutations were analyzed by using Sanger sequencing. Here, we report a new, rapid, and convenient system for screening CALR mutations without sequencing. METHODS Eighty-three bone marrow samples were obtained from 81 pati...
متن کاملFrequent EGFR mutations in malignant pleural effusion of lung adenocarcinoma
Frequent EGFR mutations in malignant pleural effusion of lung adenocarcinoma Shang-Gin Wu, Chien-Hung Gow, Chong-Jen Yu, Yih-Leong Chang, Chih-Hsin Yang, Ya-chieh Hsu, Jin-Yuan Shih*, Yung-Chie Lee, Pan-Chyr Yang 1 Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan. 2 Division of Critical Care Medicine, Department of Emergency and Critical Care Medicine, Lotung...
متن کاملCurrent two EGFR mutations in lung adenocarcinoma - case report.
Nowadays, EGFR TKIs (epidermal growth factor receptor-tyrosine kinase inhibitors) targeted therapy is well established treatment for patients with the so-called EGFR common mutations with advanced or metastatic nonsmall cell lung cancer. The efficacy for the so-called rare and especially for the very rare complex EGFR mutations is not clear. We describe a case of a 63- year-old female with meta...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Yonsei Medical Journal
سال: 2018
ISSN: 0513-5796,1976-2437
DOI: 10.3349/ymj.2018.59.1.13